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HDL Cholesterol

In the first step of the reaction, Anti Human lipoprotein antibody binds to lipoproteins other than HDL. The complexes thus formed inhibit enzyme reactions when the reagent 2 is added. Cholesterol Oxidsase and Cholesterol Esterase in the R2 react only with HDL C. Hydrogen Peroxide produced by the enzyme reaction with HDL C produces a blue colour complex upon oxidative condensation of F-DOAS and 4 Amino Antipyrine in the presence of Peroxidase. By measuring the absorbance of the Blue coloured complex, HDL C concentration is quantitated.

Biorex Diagnostics manufactures HDL reagents designed for Hitachi 917 and Olympus AU600/400/640/2700.

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Description

Product Name Details CAT.No Size
HDL Cholesterol Suitable for Hitachi 917 HIT0421A R1: 6 x 40ml R2: 4 x 20ml
HDL Cholesterol Suitable for Hitachi 917 HIT0421G R1: 6 x 20ml R2: 2 x 20ml
HDL Cholesterol Direct Suitable for use on Olympus System AU600, AU400, AU640, AU2700 OLY0421H R1: 6 x 30ml R2: 2 x 30ml

 

Product Description

The Biorex Diagnostics HDL Cholesterol Assay is an in vitro diagnostic test designed for the direct quantitative determination of high-density lipoprotein cholesterol (HDL-C) in human serum and plasma. HDL-C, often referred to as “good cholesterol,” plays a central role in lipid metabolism by mediating reverse cholesterol transport, carrying excess cholesterol from peripheral tissues back to the liver for excretion. Accurate measurement of HDL-C is essential for evaluating cardiovascular risk, assessing lipid disorders, and guiding therapeutic interventions. The Biorex HDL Cholesterol Assay provides reliable, reproducible, and clinically meaningful results, supporting effective patient management and research applications.

The assay’s direct measurement method eliminates interference from low-density (LDL) and very-low-density lipoproteins (VLDL), ensuring precise evaluation of HDL-C levels. This capability is critical in clinical scenarios where total cholesterol levels may not provide sufficient insight into lipid balance or cardiovascular risk. By delivering high-quality data, the Biorex Assay enables clinicians and laboratory professionals to make informed decisions regarding patient care, preventive measures, and treatment monitoring.

Designed for use with automated analyzers, the assay features a convenient two-step enzymatic colorimetric method, minimizing hands-on preparation and reducing the risk of errors. Its robustness and reproducibility ensure consistent performance across multiple testing sessions, laboratory instruments, and patient samples. The assay is compatible with widely used analyzers including Hitachi 917 and Olympus AU600/400/640/2700 systems, allowing seamless integration into routine laboratory workflows and high-throughput environments.

Key Features

  • Direct measurement of HDL cholesterol without LDL or VLDL interference
  • Supports cardiovascular risk assessment and management of lipid disorders
  • Two-step enzymatic colorimetric method ensures high accuracy and reproducibility
  • Fast, convenient, and ready for automated analyzer use
  • Compatible with Hitachi 917 and Olympus AU600/400/640/2700 systems

Benefits

  • Provides precise, reproducible, and clinically relevant HDL-C data
  • Facilitates accurate lipid profiling and cardiovascular risk evaluation
  • Enhances laboratory efficiency and reduces analytical errors
  • Supports patient management, preventive care, and research studies
  • Enables reliable monitoring of therapeutic interventions targeting lipid balance

Routine use of the Biorex HDL Cholesterol Assay allows laboratories to maintain high-quality performance in lipid diagnostics. Its combination of direct measurement, analytical precision, and automated compatibility makes it an indispensable tool for clinical laboratories, hospital diagnostics, preventive health screening, and cardiovascular research. By integrating this assay into daily workflows, laboratories can provide actionable data to support accurate patient assessment and improved cardiovascular health outcomes.

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Additional information

Storage

2-8°C